RNA Sequencing

RNA-Sequencing

RNA-Seq provides a precise and complete snapshot of the transcriptome and enables the identification of novel transcripts, alternative splicing and gene fusion events. RNA-Seq also provides an alternative and affordable approach for gene expression quantification and differential gene expression analysis among groups of samples.

Overview of Service:

Sequencing Strategy:  

ü  Library preparation using total RNA from frozen tissues and FFPRE tissues.

ü  Sequencing 150 x2 PE or 75x2 PE using HiSeq4000 or NextGene500 platform.

Bioinformatic analysis:

  • Gene expression analysis (≥ 20 million reads recommended)
  • Mapping, alignment, and expression level reports
  • Comparison of expression levels between samples or sample groups
  • Go enrichment analysis and KEGG pathway analysis
  • Transcriptomic data analysis (≥ 40 million reads recommended)
  • Mapping, alignment and expression level reports
  • Protein-protein interaction analysis
  • Functional annotation of transcription factors
  • Alternative splicing analysis
  • Fusion gene analysis
  • SNP/InDel analysis
Sample Requirements
Prices: Depends on the data size needed for your research, our competitive price is estimated to be $330 per sample.ta.