Single Cell DNA Sequencing
With single-cell DNA sequencing, the genomic heterogeneity of cell populations can be explored at the level of the individual cell. Genetic changes, such as point mutations and copy number variation occurring during disease and normal development processes, are profiled using the minute amounts of DNA from single cells. Applications include analysis of genetic heterogeneity within unicellular and multicellular organisms, detection of chromosomal anomalies in germ line cells, preimplantation genomic screening of embryos, and defining the genetic composition of tumors for developing more targeted therapies.
We offer the highest quality single cell sequencing services including DNA amplification, library construction, sequencing and bioinformatics analysis using our unique technologies. We use a method has high fidelity amplification, thus, avoid artifact caused by cytosine deamination (mostly C - > T). It reduces error rate to ~ 200 false SNVs per single cell from > 20,000 false SNVs per single cell from most commonly used kit, and enables the discovery of accurate somatic single nucleotide variations (SNVs) in single cells.
Sequencing Strategy
- DNA amplification using a method allows yielding ~3ug DNA from a single cell for sequencing.
- 350 bp insert DNA library
- HiSeq platform, paired-end 150 bp