Single Cell RNA Sequencing
Single-cell RNA-Seq enables the high-resolution transcriptome profiling of a single cell, and has broad utility for investigating developmental processes and gene regulatory networks, and for revealing heterogeneous gene expression patterns within cell cultures, tissues, and organs. Applications include profiling gene expression changes during stem cell differentiation, organ development, or tumor progression, and characterizing the response of subpopulations of cells to environmental signals and conditions. We offer the highest quality of single-cell RNA-Seq service includes amplification, library construction, sequencing, and bioinformatics analysis.
Sequencing Strategy
- SMARTer kit for single cell RNA amplification.
- 250-300 bp insert cDNA library
- Sequencing using HiSeq4000 or NextGene500 platform.
Data Quality Guarantee: Q30 ≥ 80%
Turnaround Time: 25 working days
Recommended Sequencing Depth
- ≥ 20 million reads for gene expression studies
- ≥ 40 million reads for transcriptome studies
Sample Requirements
• We accept fresh single cells and laser captured single cells from FFPE slides.
• Circulating tumor cells (CTC) may be purified using our Thermoresponsive CTC purification system and used for RNAseq.
• Single cells should be stored in one of the two ways 1) in lysis buffer with RNase inhibitor from SMARTer kit (Clontech) or 2) in 1X PBS buffer (excluding calcium and magnesium) containing RNase inhibitor in a total volume of ≤ 2 μl. The stored cell should be frozen in liquid nitrogen and shipped out with dry ice.
Price: Depends on the number of samples needing for WGS, the prices start at $1195 per sample for WGS of 30X and 90Gb data.