NanoVelcro Circulating Tumor Assay – Prism Genomic Medicine

CTC technology provides an alternative to biopsies for areas with low tissue resources, and advanced studies using the isolated CTCs. Working with our partners, we provide circulating tumor cell (CTC) enumeration and CTC sequencing services.

1^st-platform: NanoVelcro Chip, composed of a silicon nanowire substrate and an overlaid microfluidic chaotic mixer, allows capture CTCs for CTC enumeration and for CTC biomarker immunostaining.
2^nd-platform: NanoVelcro-LCM technology can be used to enrich/identify/isolate CTCs with single-cell precision.
3^rd-platform: Thermoresponsive CTC Purification System: By grafting thermoresponsive polymer brushes onto nanosubstrates, CTCs can be captured and released of CTCs at 37 and 4°C, respectively. The temperature-dependent conformational changes of polymer brushes can effectively alter the accessibility of the capture agent on nanosubstrates, allowing for rapid CTC purification with desired viability and molecular integrity.

Purified CTCs can be subjected to a variety of molecular analyses, including mutational analysis by RT-PCR and Sanger sequencing, as well as NGS at both whole exome and whole genome levels