Sample Requirements

SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	PURITY (NANODROPTM/AGAROSE GEL)
	STRONGLY RECOMMENDED	REQUIRED
Genomic DNA	≥ 2 μg	≥ 1 μg	≥ 20 μL	≥ 50 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination
PCR products of   single-cell whole genome	≥ 2 μg	≥ 1 μg	≥ 20 μL	≥ 50 ng/μL	Fragments should be longer than 500 bp
FFPE*	≥ 3 μg	≥ 1.5 μg	–	–	Fragments should be longer than 1500 bp

SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	PURITY (NANODROPTM/AGAROSE GEL)
	STRONGLY RECOMMENDED	REQUIRED
Genomic DNA	≥ 1 μg	≥ 500 ng	≥ 20 μL	≥ 50 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination
PCR products of single-cell whole genome	≥ 1 μg	≥ 500 ng	≥ 20 μL	≥ 50 ng/μL	Fragments should be longer than 500 bp
FFPE	≥ 2 μg	≥ 1 μg	–	–	Fragments should be longer than 1500 bp

LIBRARY TYPE	SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	PURITY (NANODROPTM/AGAROSE GEL)
		STRONGLY RECOMMENDED	REQUIRED
≤ 500 bp Insert	Genomic DNA	≥ 1.4 μg	≥ 700 ng	≥ 20 μL	≥ 50 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination
	Mitochondrion/ Chloroplast DNA	≥ 1.6 μg	≥ 800 ng	≥ 20 μL	≥ 50 ng/μL
Genotyping by Sequencing	Genomic DNA	≥ 500 ng	≥ 300 ng	≥ 10 μL	≥ 50 ng/μL
2 Kb Insert	Genomic DNA	≥ 30 μg	≥ 15 μg	≥ 20 μL	≥ 50 ng/μL
5 Kb Insert	Genomic DNA	≥ 30 μg	≥ 15 μg	≥ 20 μL	≥ 50 ng/μL
10 Kb Insert	Genomic DNA	≥ 50 μg	≥ 25 μg	≥ 20 μL	≥ 50 ng/μL
> 10 Kb Insert	Genomic DNA	≥ 80 μg	≥ 40 μg	≥ 20 μL	≥ 50 ng/μL

 

LIBRARY TYPE	SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	PURITY   (NANODROPTM/AGAROSE GEL)
		STRONGLY RECOMMENDED	REQUIRED
≤ 500 bp Insert	Genomic DNA	≥ 1.6 μg	≥ 800 ng	≥ 20 μL	≥ 50 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination
Meta Library	Genomic DNA	≥ 1.6 μg	≥ 800 ng	≥ 20 μL	≥ 50 ng/μL	Fragments should be longer than 500 bp
PCR-Free Library	Genomic DNA	≥ 10 μg	≥ 5 μg	≥ 20 μL	≥ 50 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination
	PCR Products*	≥ 400 ng	≥ 200 ng	≥ 10 μL	≥ 20 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination
	PCR Products*	≥ 200 ng	≥ 100 ng	≥ 10 μL	≥ 20 ng/μL	OD260/280 = 1.8 – 2.0,   no degradation, no contamination

 

SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	PURITY (NANODROPTM/ AGAROSE GEL)
	STRONGLY RECOMMENDED	REQUIRED
ChIP-Seq DNA	≥ 100 ng	≥ 50 ng	≥ 10 μL	≥ 50 ng/μL	Main peak of 100 bp – 500 bp

 

LIBRARY TYPE	SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	RNA INTEGRITY NUMBER (AGILENT 2100)	PURITY （NANODROPTM）
		STRONGLY RECOMMENDED	REQUIRED
Eukaryotic RNA-Seq	Total RNA (Animal)	≥ 2.6 μg	≥1.3 μg	≥ 20 μL	≥ 50 ng/μL	≥ 6.8, smooth base line	OD260/280 = 1.8 – 2.2, OD260/230 ≥ 2.0,   no degradation, no contamination
	Total RNA (Plant and Fungus )	≥ 2.6 μg	≥ 1.3 μg	≥ 20 μL	≥ 50 ng/μL	≥ 6.3, smooth base line
Prokaryotic RNA-Seq	Total RNA	≥ 6 μg	≥ 3 μg	≥ 20 μL	≥ 50 ng/μL	≥ 6.0, smooth base line

 

LIBRARY TYPE	SAMPLE TYPE	AMOUNT (QUBIT®)		VOLUME	CONCENTRATION	RNA INTEGRITY NUMBER (AGILENT 2100)	PURITY （NANODROPTM）
		STRONGLY RECOMMENDED	REQUIRED
Eukaryotic small RNA Sequencing	Total RNA (Animal)	≥ 6 μg	≥ 3 μg	≥ 20 μL	≥ 50 ng/μL	≥ 8, smooth base line	OD260/280 = 1.8 – 2.2, OD260/230 ≥ 2.0,   no degradation, no contamination
	Total RNA (Plant and Fungus )	≥ 6 μg	≥ 3 μg	≥ 20 μL	≥ 50 ng/μL	≥ 7.5, smooth base line

 

DATA AMOUNT	VOLUME REQUIREMENT*
< 30 G	≥ 10 μL
≥ 30 G	≥ 20 μL

*High concentration samples should be diluted before delivery

(2) Library concentration: library concentration quantified by Qubit® 2.0 (Life Technologies): ≥ 0.5 ng/uL

(3) Insert size: dilute to 1 ng/µL before checking the insert size by Agilent 2100 Bioanalyzer.

• Insert size: insert + adapters (120 bp) ± 50 bp (Does not apply to small RNA library)

• Main peak present, no multiple peaks, no adapter contamination and no primer dimers.

(4) Library concentration quantified by Q-PCR:

PLATFORM	CONCENTRATION REQUIREMENT
HiSeq 2500	2 nM – 30 nM
MiSeq	4 nM – 30 nM
HiSeq X	3 nM – 30 nM

SAMPLE LABELING RECOMMENDATIONS

1. It is important to prevent the sample labels from being dissolved by solvents and from falling off the tubes. We strongly recommend you use a waterproof marker pen to write directly on the tube wall or lid. You can also write the sample information on a paper/plastic label, stick the label onto the tube wall, and then secure the label to the tube by wrapping with clear, adhesive tape (e.g. Scotch tape) completely around the tube.
2. Please fill out and attach the Sample Submission Form in the email before shipping the samples. Please make sure that the sample information on the Sample Submission Form matches the labels on the tubes.

SAMPLE PACKING RECOMMENDATIONS

1. For DNA and RNA samples, we recommend 1.5 ml or 2 ml screw-cap DNase- and RNase-free microcentrifuge tubes. Please use Parafilm to seal each tube before packaging. Novogene does not recommend shipping samples dissolved in organic solvents (such as absolute ethanol or isopropanol) because the solvents may cause leakage of the samples, which can result in cross-contamination between samples. If it is unavoidable to ship samples in organic solvents, please use screw-cap tubes and seal the opening of the tube with at least 10 layers of Parafilm.
2. In order to avoid crushing during shipping, we highly recommends placing the sample tubes in a container such as a 50-ml tube or a box with interior racks/holders. Cotton and absorbent papers can be used to prevent tubes from moving around inside the container.
3. RNA samples should be kept in dry ice during shipment. Genomic DNA samples should be kept in blue ice during shipment. Saliva samples should be shipped at room temperature.
4. In order to stick with our high quality control standards, 96-well plates and PCR stripe tubes are NOT acceptable containers for your sample shipping. The only container we allow for sample shipping is 1.5 ml or 2 ml tube. (See picture below).

COMPLETING THE SAMPLE SUBMISSION FORM

A Sample Information Form must be submitted for each sequencing service project. All information on the forms should be filled out carefully. Please submit the completed ELECTRONIC COPY via email to our local sales representative and enclose a HARD COPY in the shipment. In both copies, please make sure you mark the samples summary (Sample types and number) at the top of the Form (Fig. 13)

SHIPPING SAMPLES TO PRISM

Disclaimer: The information below only constitutes a recommendation for shipping samples classified as “non-regulated materials” to our facility. At the time this document was prepared, gDNA/total RNA was not defined as a diagnostic specimen in the International Air Transport Association (IATA) packing instructions, and therefore no special packaging requirements are listed. Due to continuing changes in regulations, customers should always check with their safety office and/or shipping department to ensure regulatory compliance.

1. Ensure that all samples conform to our quality standards and that they are prepared and packaged according to the guidelines given above.
2. Please make sure to notify a Prism representative and to send the required documents before shipping your samples.

Address:

Prism Genomic Medicine, Inc.
6655 Travis Street, Suite #840
Houston, Texas, 77030

Telephone: 832 787 1886

TRANSPORTATION OPTIONS

DNA	Lyophilize the DNA for shipping at ambient temperature
	Pack with ice packs/blue ice (2-8 °C)
	Use the cold-chain transportation system (2-8 °C) of the courier
	DNA Stable (Liquid format, Biomatrica)
	Pack in dry ice (-60 °C – -80 °C)
RNA	Lyophilize the RNA for shipping at 2-8 °C or ambient temperature
	Suspend RNA in 75% ethanol and ship on dry ice
	RNAstable (Biomatrica)
	Pack in dry ice (-60 °C – -80 °C)

Note:

a. It is highly recommended that RNA samples be shipped in dry ice packaging. Other packaging/transportation methods may add impurities or cause slight degradation of the RNA.

b. The quantity of dry ice and ice bags needed varies with the season (i.e., room temperature), transit time, and the thickness of Styrofoam box and receptacle. Please contact your local courier office for estimated transit time. Normally, dry ice is consumed (sublimates) at a rate of 5 kg (11 pounds) per day.